ANTI-STAT3; CLONE 4N11; ZOOMAB RABBIT M

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Stat3 in SK-N-MC and NIH3T3 cell lysate.Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Stat3 in human colon, human liver and human skin tissue sections.Immunocytochemistry Analysis: A 1:150 dilution from a representative lot detected Stat3 in HUVEC, HeLa, A431, and NIH 3T3 cells.

Anti-Stat3, clone 4N11 ZooMAb, Cat. No. ZRB1004, is a recombinant Rabbit Monoclonal Antibody that detects Stat3 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.Learn more about ZooMAb here.

Immunogen

KLH-conjugated linear peptide corresponding to 11 amino acids from the C-terminal region of human Signal transducer and activator of transcription 3 (Stat3).

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Specificity

Clone 4N11 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Stat3. It targets an epitope with in 11 amino acids from the C-terminal region.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Target description

Signal transducer and activator of transcription 3 (UniProt: P40763; also known as Stat3, Acute-phase response factor) is encoded by the STAT3 (also known as APRF) gene (Gene ID: 6774) in human. STAT3 is expressed in most cell types and is activated by multiple cytokines and growth factors. It is a latent cytoplasmic transcription factor that has a dual function of signal transduction and activation of transcription. It mediates cellular responses to interleukins and growth factors. STAT3 shuttles between the nucleus and the cytoplasm. STAT3 is activated by phosphorylation at Tyrosine 705, which induces dimerization, nuclear translocation and DNA binding. A motif comprising of amino acids 150-162 is essential for its nuclear import. In the nucleus it recruits coactivators, such as NCOA1 or MED1, to the promoter region of the target genes. It binds to DNA as either a homodimer or as a heterodimer with STAT1. Constitutively activated STAT3 is reported in a number of human cancers. STAT3 regulates inflammatory response by regulating differentiation of naive CD4+ T-cells into T-helper Th17 or regulatory T-cells. STAT3 may also play an apoptotic role by transactivating BIRC5 expression under LEP activation. Ad cytoplasmic STAT3 is shown to repress macroautophagy by inhibiting EIF2AK2/PKR activity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.