Application

Antibody is suitable for immunoblotting, immunoprecipitation, and indirect immunofluorescence.immunocytochemistry.

Anti-VSV-G antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Chromatin immunoprecipitation (1 paper)Western Blotting (1 paper)Western blotting following immunoprecipitation (1 paper)

Biochem/physiol Actions

Vesicular stomatitis virus G (VSV G) is capable of stimulating cell-cell fusion upon low pH stimulation.The transmembrane region of VSV G protein is highly essential for the virus transition from the hemi-fusion to full fusion state.

General description

Vesicular stomatitis viruses (VSV) belongs to the Vesiculovirusgenusof the Rhabdoviridae family. Vesicular stomatitis virus G (VSV G) protein is considered to be the early recognized type III viral fusion protein.

The Anti-VSV-G rabbit antibody reacts with amino- and carboxyl-terminal VSV-G tagged fusion proteins.

Immunogen

synthetic peptide corresponding to amino acids 497-511 (N-Tyr-Thr-Asp-Ile-Glu-Met-Asn-Arg-Leu-Gly-Lys-C) of the Vesicular Stomatitis Virus glycoprotein (VSV-G), conjugated to maleimide activated KLH via a cysteine residue added at the carboxyl terminus. The antibody recognizes the VSV-G tag at both the C-terminus and N-terminus.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.