MONOCLONAL ANTI-TUBULIN POLYGLUTAMYL

Application

Monoclonal anti-tubulin, polyglutamylated antibody can be used in immunoblotting, immunocytochemistry and ELISA. It can also be used as primary antibodies (diluted 1:1000) in western blotting.

Monoclonal Anti-Tubulin, Polyglutamylated antibody produced in mouse has been used in:immunoprecipitationimmunohistochemistryimmunofluorescence

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.Immunocytochemistry (1 paper)Western Blotting (1 paper)

Biochem/physiol Actions

Microtubule plays an important role in DNA segregation.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tubulin is a 100kD protein that is present in all cells as a heterodimer of two similar polypeptides α and β which assemble to form microtubules. Proper organization of microtubules is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions. Monoclonal anti-tubulin, polyglutamylated antibody can be used as primary antibody in colocalization experiments. This antibody can also be used for studies involving the inhibition of flagellar motility in permeabilized sperm models. Mouse anti-tubulin, polyglutamylated antibody reacts specifically with the epitope present in the C-terminal region of α and β tubulins (glutamylated motif at amino acids 445-457 of α-tubulin).

Tubulin is a cellular colchicine-binding protein.

Immunogen

purified sea urchin (Lytechinus pictus) sperm axonemal proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide