Anti-Alpha-Tubulin antibody Mose monocl

Code: T8203-100UL D2-231

Application

Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.

The antibody has been used in various immunochemical techniques including immun...


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€498.00 100UL
€612.54 inc. VAT

Application

Anti-α-Tubulin antibody, Mouse monoclonal has been used in western blotting.

The antibody has been used in various immunochemical techniques including immunoprecipitation, immunocytochemistry, and immunohistochemistry.

Biochem/physiol Actions

Gene mutation of tubulin α-1A (TUBA1A) is observed in perisylvian asymmetrical polymicrogyria, polymicrogyria-like cortical dysplasia, and microlissencephaly in foetal cases. Alteration in the expression of the gene impairs neural migration and causes lissencephaly (LIS).

Tubulin is the major building block of microtubules, which is a structural and mobile element in mitosis, intracellular transport, flagellar movement and the cytoskeleton. Monoclonal antibodies recognizing α-tubulin, together with monoclonal antibodies to other tubulin types (α-tubulin isotypes I, II, and III, tyrosine tubulin and acetylated- α -tubulin) provide a specific and useful tool in studying the intracellular distribution of tubulin, and the static and dynamic aspects of the cytoskeleton. In addition, the various tubulin forms have been implicated in cancerous processes and are thus suggested as potential targets for cancer therapy.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anti-α-Tubulin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma AA13 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with rat brain tubulin.

Tubulin α 1a (TUBA1A) is a member of α-tubulin gene family. It is encoded by the gene mapped to human chromosome 12q13.12. Tubulin α 1a is a vital structural subunit of microtubules that are temporarily expressed in almost all post-mitotic neurons during neuronal development.The encoded protein structure contains core of two β-sheets enclosed by α-helices. TUBA1A is characterized with an amino-terminal domain with the guanine nucleotide-binding region, an intermediate domain associated with interprotofilament lateral contacts and the modulation of tubulin curvature, and a carboxy-terminal domain that contains region involved in interacting with microtubule-associated proteins (MAPs) and molecular motors, such as kinesins and dynein.

Immunogen

rat brain tubulin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Target description

Tubulin is a cylindrical, filamentous structure is present inalmost all eukaryotic cells.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
cloneAA13, monoclonal
concentration~2.0 mg/mL
conjugateunconjugated
enhanced validationindependentLearn more about Antibody Enhanced Validation
formbuffered aqueous solution
Gene Informationhuman ... TUBA1A(7846)mouse ... Tuba1a(22142)rat ... Tuba1a(64158)
isotypeIgG1
mol wtantigen ~50 kDa
packagingantibody small pack of 25 µL
Quality Level200
shipped indry ice
species reactivityhuman, mouse, rat
storage temp.−20°C
technique(s)indirect ELISA: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: 1-2 µg/mL using Neuro-2a cell extract, immunohistochemistry: suitable
UniProt accession no.Q71U36
This product has met the following criteria to qualify for the following awards:



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