Anti-NDE1 Mouse mAb (2G11-1C11)

Analysis Note

Positive ControlHeLa cells

Application

ELISA (see coments)Immunoblotting (1-4 g/ml)Immunocytochemistry (10 g/ml)

General description

This Anti-NDE1 Mouse mAb (2G11-1C11) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of NDE1.

Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa NDE1 protein.

Recognizes the ~40-45 kDa NDE1 protein in HeLa cells.

Immunogen

Full-length, recombinant, human NDE1 (aa 1-336), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Bradshaw, N.J., et al. 2009. Neurosci. Lett.449, 228.

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)