Anti-MAP4K4 Mouse mAb (4A5)

Analysis Note

Positive ControlK-562 cells, Human breast cancer tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3 g/ml)

General description

Purified mouse monoclonal antibody. Recognizes the ~140 kDa MAP4K4 protein.

This Anti-MAP4K4 Mouse mAb (4A5) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of MAP4K4.

Recognizes the ~140 kDa MAP4K4 protein in K-562 cells and human breast cancer tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids of 611-710 human MAP4K4, expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)