Analysis Note

Positive ControlPC-12 cells, Jurkat cells, HepG2 cells, NIH3T3 cells, Raw 264.7 cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)

General description

Recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells.

Purified mouse monoclonal antibody. Recognizes the ~30-35 kDa FGF8 protein.

Anti-FGF8, mouse monoclonal, clone 2A10, recognizes the ~30-35 kDa FGF8 protein in PC-12, Jurkat, HepG2, NIH3T3, and Raw 264.7 cells. It is validated for use in ELISA and Western blotting.

Immunogen

Full-length, recombinant, human FGF8 (aa 65-133), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)