Analysis Note

Positive ControlHuman tonsil tissue

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Paraffin Sections (3 g/ml)

General description

Purified mouse monoclonal antibody. Recgonizes the FGF21 protein.

Recgonizes the FGF21 protein in human tonsil tissue.

Anti-FGF21, mouse monoclonal, clone 2F11, recognizes the FGF21 protein in human tonsil tissue. It is validated for use in ELISA, Western blotting, and Immunohistochemistry (paraffin sections).

Immunogen

Full-length, recombinant human FGF21 (aa 30-210), expressed as a GST fusion protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)