Analysis Note

Negative Control293T cells

Positive ControlHuman testes tissue, HeLa cells

Application

ELISA (see comments)Immunoblotting (1-5 g/ml)Immunocytochemistry (10 g/ml)Paraffin Sections (3 g/ml)

General description

Recognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells.

Purified mouse monoclonal antibody. Recognizes the ~60 kDa PIP5K3 protein.

This Anti-PIP5K3 Mouse mAb (6C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of PIP5K3.

Immunogen

A recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Tsuruta, F., et al. 2009. J. Cell Biol.187 279.

Physical form

In PBS, pH 7.2.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Regulatory Review (Z)