Anti-TORC2 (454-607) Rabbit pAb

Analysis Note

Positive ControlPrimary rat hepatocytes, HepG2, NIH3T3, Min6, and FAO cells

Application

Immunoblotting (1:2000, see comments)Immunoprecipitation (1:100, see comments)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~79-85 kDa TORC2 protein.

This Anti-TORC2 (454-607) Rabbit pAb is validated for use in Immunoblotting, Immunoprecipitation for the detection of TORC2 (454-607).

TORCs (Transducers Of Regulated cAMP Response Element-Binding (CREB) protein) are members of a conserved family of co-activators that enhance CRE-dependent transcription by a phosphorylation-independent interaction with the bZIP DNA binding/dimerization domain of CREB. Studies suggest that TORC phosphorylation and nuclear/cytoplasmic shuttling play an important role in the regulation of gluconeogenesis by cAMP.

Recognizes the ~79-85 kDa TORC2 protein in primary hepatocytes, HepG2, NIH3T3, Min6, and FAO cells.

Immunogen

a recombinant protein consisting of amino acids 454-607 of mouse TORC2 fused to GST

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

This antibody has been used to detect endogenous TORC2 by immunoblotting. For immunoprecipitation, 4 µl antibody in a total volume of 400 µl has been tested. May also detect a protein at ~60 kDa, which may be a degradation product of TORC2. Antibody should be titrated for optimal results in individual systems.

Screaton, R.A., et al. 2004. Cell119, 61.

Packaging

50 µL in Glass bottle

Physical form

Undiluted serum.

Reconstitution

For long-term storage, aliquot and freeze (-20°C). In the case of storage at -20°C, avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)