Application

The Anti-6-His antibody has been used in western blotting.

Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

Biochem/physiol Actions

The antibody recognizes the His-tag fused to the carboxy- termini of targeted proteins in transfected or transformed cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

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General description

The FLAG epitope, is an eight amino acid protein with an enterokinase-cleavage site.

Immunogen

Synthetic peptide: 18XHis conjugated to KLH

Physical form

Rabbit IgG in pH7.3 PBS, 0.05% NaN₃, 50% Glycerol.

Specificity

The antibody detects transfected proteins contanining 6×His tag.