Application

The antibody is recommended to use in various immunological techniques, including Immunoblot (~24 kDa) and immunofluorescence. Detection of the TIMP2 band by Immunoblotting is specifically inhibited by the immunogen.

Biochem/physiol Actions

The MMPs activity inhibition is mediated directly by blocking the MMPs catalytic zinc ion with the TIMPs N-terminal cysteine residue.1-2 TIMP2 is also highly studied for it MMP-independent effects including cell cycle regulation 2-4 and its involvement in several cancers progression and invasiveness (e.g. renal cell carcinoma3, non-small cell lung cancer (NSCLC)5 and Gastric Cancer6).Due to the pronounced inhibitory activity, the TIMPs are considered as a potential target in designing new inhibitor variants with altered specificity for MMPs and other zinc-dependent extracellular matrix (ECM) proteins.1-2

Disclaimer

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

TIMP2- Tissue Inhibitor of Metalloproteinases 2, also known as CSC-21K, is a member of the TIMP family of matrix metalloproteinases (MMPs) natural inhibitors. These proteins are involved in regulation of activated MMPs and in extracellular matrix degradation. the TIMPs family consists of 4 members which act in a non-selective manner.

Immunogen

Recombinant TIMP2 protein.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Specificity

Monoclonal Anti-TIMP2 specifically recognizes human TIMP2.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.