Application

Monoclonal Anti-ALIX/PDCD6IP antibody has been used in immunoblotting immunoprecipitation immunofluorescence flow cytometry

Biochem/physiol Actions

ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X) plays a role in ESCRT (endosomal sorting complexes required for transport) pathway. It is involved in the abscission stage of cytokinesis, in intralumenal endosomal localization, and in enveloped virus budding. Overexpression of this protein can block apoptosis. In addition, ALIX binds to PDCD6, a protein required for apoptosis, in a calcium-dependent manner. ALIX also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of ALIX and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death.

Disclaimer

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General description

Anti-ALIX/PDCD6IP antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma ALIX-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide corresponding to an internal region of human ALIX/ PDCD6IP, conjugated to KLH. ALIX (Apoptosis-linked gene 2 (ALG-2)-interacting protein X), also named Programmed Cell Death 6 interacting protein (PDCD6IP).

Immunogen

peptide corresponding to an internal region of human ALIX/ PDCD6IP

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.