PhosphoDetect Anti-Smad3 (pSer423/425) Rabbit mAb (C25A9)

Application

Immunoblotting (1:1000)Immunoprecipitation (1:50)

General description

This PhosphoDetect™ Anti-Smad3 (pSer^423/425) Rabbit mAb (C25A9) is validated for use in Immunoblotting, Immunoprecipitation for the detection of Smad3 (pSer^423/425).

Purified rabbit monoclonal antibody generated by immunizing rabbits with the specified immunogen and fusing splenocytes with an immortal cell line. Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425.

Recognizes the ~52 kDa Smad3 protein phosphorylated at Ser^423/425 in HT-1080 cells treated with TGF-β1.

Immunogen

a synthetic phosphopeptide corresponding to amino acids surrounding the Ser ^423/425 phosphorylation sites of Smad3, coupled to KLH.

Legal Information

TWEEN is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Attisano, L., et al. 2002. Science 296, 1646.Moustakas, A., et al. 2001. J. Cell Sci. 114, 4359.Wrana, J. 2000. Science 23, 1.

Does not cross-react with other Smad proteins. For immunoblotting incubate the membrane with antibody diluted with TBS containing 5% BSA, 0.1% Tween^®-20 detergent overnight at 4°C with gentle shaking. Please note that this is a rabbit monoclonal antibody and requires the use of an anti-rabbit secondary antibody. The immunogen sequence is 100% conserved in mouse, rat, monkey, bovine, xenopus, and zebrafish Smad3, but cross-reactivity has not been tested. Antibody should be titrated for optimal results in individual systems.

Packaging

50 µL in Plastic ampoule

Physical form

In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol pH 7.5.

Warning

Toxicity: Standard Handling (A)