Anti-Green Fluorescent Protein (26-39) Rabbit pAb

Application

Immunoblotting (1:2000)r>Immunocytochemistry (1:200)

General description

Anti-Green Fluorescent Protein (26-39), rabbit polyclonal, recognizes the ~28 kDa GFP and some GFP fusion proteins. It is validated for use in Western blotting and immunocytochemistry.

Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~28 kDa GFP and some GFP fusion proteins.r>

Recognizes the ~28 kDa GFP and some GFP fusion proteins.

Immunogen

Aequora victoria

a synthetic peptide corresponding to amino acids 26-39 of Aequorea victoria wild-type green fluorescent protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Cubitt, A.B., et al. 1995. Trends Biochem. Sci.20, 448.

This sequence is completely conserved between GFP and the modified and enhanced Blue, Green, and Yellow FP variants. This antibody has been successfully used in immunoblotting and immunocytochemistry protocols to detect GFP and fusion proteins. It detects a ~28 kDa protein that corresponds to GFP in immunoblots of protein extract from cells transfected with, and overexpressing, GFP isoforms. Antibody should be titrated for optimal results in individual sytems.

Packaging

100 µL in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 1 mg/ml BSA.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)