Anti-Opioid # Receptor (384-

Analysis Note

Positive ControlRat caudate putamen or spinal cord (dorsal horn)

Application

Frozen Sections (1:500-1:1000, Cy3 technique; 1:3000-1:6000, HRP)r>Immunoblotting (1:2000-1:2500; see application references)r>Immunofluorescence (1:100-1:200)r>Immunoprecipitation (see comments)

General description

Rabbit polyclonal antibody supplied as lyophilized, undiluted serum. Recognizes the ~44-45 kDa opioid µ receptor protein.

Recognizes the ~44-45 kDa opiod µ receptor in caudate putamen and the dorsal horn of the spinal cord.

This Anti-Opioid µ Receptor (384-398) Rabbit pAb is validated for use in Frozen Sections, Immunoblotting, IF, IP for the detection of Opioid µ Receptor (384-398).

Immunogen

a synthetic peptide corresponding to amino acids 384-398 of rat opioid µ receptor

Rat

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

The specificity was determined by immunolabeling of transfected cells, immunoblotting analysis, and immunoisolation studies. Antibody specificity was examined in the rat caudate putamen and dorsal horn of the spinal cord. Staining is completely eliminated by pre-treatment of antibody with immunogen peptide at a concentration of 10 µg/ml. This antibody has also been reported to work for immunopercipitation. Antibody should be titrated for optimal results in individual systems.

Zaki, P.A., et al. 1996. Annu. Rev. Pharmacol. Toxicol.36, 379.Arvidsson, U., et al. 1995. J. Neurosci.15, 3328.Kieffer, B.L. 1995. Cell. Mol. Neurobiol.15, 615-635.Childers, S.R. 1991. Life Sci.48, 1991.

Packaging

100 µL in Plastic ampoule

Physical form

Undiluted serum.

Reconstitution

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Resulting reconstituted solution contains ≤0.1% sodium azide. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C).

Warning

Toxicity: Highly Toxic (H)