Monoclonal Anti-p53 antibody produced in mouse, clone DO-1, purified immunoglobulin, buffered aqueous solution

Code: P6874-200UL D2-231

Application

Anti-p53 antibody, Mouse monoclonal has been used in:semi-dry western blotting immunoblotting immunofluorescence enzyme-linked immunosorbent assay (ELISA)immunocy...


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Your Price
€686.00 200UL
€843.78 inc. VAT

Application

Anti-p53 antibody, Mouse monoclonal has been used in:semi-dry western blotting immunoblotting immunofluorescence enzyme-linked immunosorbent assay (ELISA)immunocytochemistryimmunohistochemistryimmunoprecipitationflow cytometry chromatin immunoprecipitation (ChIP) assay

Biochem/physiol Actions

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.

Immunogen

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Specificity

Mouse anti-(p53) antibody reacts specifically with (p53) if human.

antibody formpurified immunoglobulin
antibody product typeprimary antibodies
biological sourcemouse
cloneDO-1, monoclonal
concentration~2 mg/mL
conjugateunconjugated
formbuffered aqueous solution
Gene Informationhuman ... TP53(7157)
isotypeIgG2a
mol wtantigen ~53 kDa
Quality Level200
shipped indry ice
species reactivityhuman
storage temp.−20°C
technique(s)indirect ELISA: suitable, immunocytochemistry: suitable, western blot: 0.2-0.4 µg/mL using total cell extract of A431 cells, microarray: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable
UniProt accession no.P04637
This product has met the following criteria to qualify for the following awards:



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