Anti-BRCA1 (Ab-2) Mouse mAb (MS13)

Analysis Note

Positive ControlMCF7 cells

Application

Immunoblotting (2 µg/ml, chemiluminescence; see application references)r>Immunofluorescence (see application references)r>Immunoprecipitation (see application references)r>Paraffin Sections (see application references)

General description

Anti-BRCA1 (Ab-2), mouse monoclonal, clone MS13, recognizes ~220 kDa BRCA1 in MCF-7 cells. It is validated for Western blotting, immunofluorescence, immunoprecipitation, and for paraffin sections.

Recognizes the ~220 kDa BRCA1 protein in MCF-7 cells. Does not react with blood group antigens or growth factor receptors such as EGFR. Sold under license of U.S. Patent 5,753,441 and 6,162,897.

Purified mouse monoclonal antibody generated by immunizing mice with the specified iummunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~220 kDa BRCA1 protein.

Immunogen

Human

recombinant, human BRCA1

Epitope: within amino acids 1-304

Legal Information

Sold under license of U.S. Patents 5,753,441 and 6,162,897.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Scully, R., et al. 1996. Science272, 123.Gudas, J.M., et al. 1996. Cell Growth Differ.7, 717.Vaughn, J.P., et al. 1996. Cell Growth Differ.7, 711.Goldgar, D.E. and Reilly, P.R. 1995. Nat. Genet.11, 113.Merajver, S.D., et al. 1995. Clin. Can. Res.1, 539.Merajver, S.D., et al. 1995. Nat. Genet.9, 439.Struewing, J.P., et al. 1995. Nat. Genet.11, 198.Thompson, M.E., et al. 1995. Nat. Genet.9, 444.Futreal, P.A., et al. 1994. Science266, 120.Miki, Y., et al. 1994. Science266, 66.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Warning

Toxicity: Standard Handling (A)