Analysis Note
Positive ControlCell lines transfected with δNp73
Application
Immunoblotting (1 g/ml, chemiluminescence)
General description
Purified mouse monoclonal antibody. Recognizes the ~73 kDa p73 protein lacking the N-terminal transactivation domain, δpNp73.
Recognizes the ~73 kDa δNp73 protein.
This Anti-δNp73 Mouse mAb (38C674) is validated for use in Immunoblotting for the detection of δNp73.
Immunogen
a synthetic peptide (LYVGDPARHLAT) corresponding to amino acids 2-13 of human δNp73
Human
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Does not cross-react with full-length p73. Antibody should be titrated for optimal results in individual systems.
Levrero, M., et al. 2000 J. Cell Sci.113, 1661.Yang, A., et al. 2000. Nature404, 99.Ueda, Y., et al. 1999. Oncogene18, 4993.Kaghad, M., et al. 1997. Cell90, 809.
Packaging
100 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In PBS, 0.2% gelatin.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Standard Handling (A)
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