Analysis Note

Positive ControlA549 or MCF7 cells or breast carcinoma tissue

Application

Immunoblotting (2 g/ml, chemiluminescence)Immunofluorescence (2.5 g/ml)Immunoprecipitation (1 g/reaction, see application references)Paraffin Sections (2.5 g/ml, heat pre-treatment required)

General description

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 and MCF-7 cells and breast carcinoma tissue.

Purified mouse monoclonal antibody. Recognizes the ~90 kDa (apparent MW) MDM2 protein.

This Anti-MDM2 (Ab-5) Mouse mAb (4B2C1.11) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-5).

Immunogen

recombinant, human MDM2

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Marchetti, A., et al. 1995. J. Pathol.175, 31.Barak, Y., et al. 1993. EMBO. J.12, 461.Ladanyi, M., et al. 1993. Cancer Res.53, 16.Leach, F.S., et al. 1993. Cancer Res.53, 2231.Oliner, J.D., et al. 1993. Nature362, 857.Momand, J., et al. 1992. Cell69, 1237.Oliner, J.D., et al. 1992. Nature358, 80.Fakharzadeh, S.S., et al. 1991. EMBO J.10, 1565.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol, pH 7.5.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)