Analysis Note

Positive ControlSKBR-3 or MDA-231 cells or colon carcinoma tissue

Application

Frozen Sections (1-2 g/ml)Immunoblotting (1-2 g/ml)Immunofluorescence (0.5 g/ml)Immunoprecipitation (2 g/mg lysate)Paraffin Sections (1-2 g/ml)

General description

This Anti-p53 (Ab-12) (Pantropic) Mouse mAb (DO-7) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of p53 (Ab-12) (Pantropic).

Recognizes the ~53 kDa wild-type p53 and mutant p53 protein in SKBR-3 and MDA-231 cells and in colon carcinoma tissue.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen. Recognizes the ~53 kDa wild-type and mutant forms of p53.

Immunogen

Epitope: within amino acids 37-45 of p53

recombinant, human p53 expressed in E. coli

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Greenblatt, M.S., et al. 1994. Cancer Res.54, 4855.Lane, D.P. 1992. Nature358, 15.

Exhibits nuclear staining in tumor cells. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)