Analysis Note

Negative ControlNormal skin tissue or HepG2 cells

General description

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.

This clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)