Analysis Note

Positive ControlDaudi cells

Application

Immunoblotting (1-2 g/ml, chemiluminescence)

General description

Recognizes the ~85 kDa IKKα protein in Daudi cells.

This Anti-IKKα Mouse mAb (14A231) is validated for use in Immunoblotting for the detection of IKKα.

Immunoaffinity purified mouse monoclonal antibody. Recognizes the ~85 kDa IKKα protein.

Immunogen

Human

a recombinant protein consisting of full-length human IKKα/IKK1 fused to a His Tag™ sequence

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zandi, E., et al. 1998 Science281, 1360.Didonato, J.A., et al. 1997 Nature388, 548.Lee, F.S., et al. 1997 Cell88, 213.Mercurio, F., et al. 1997 Science278, 860.Regnier, C.H., et al. M. 1997 Cell90, 373.Verma, I. and Stevenson, J.K. 1997 Proc. Nat. Acad. Sci. USA94, 11758.Verma, I.M., et al. 1995 Genes. Dev.9, 2723.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS, 0.2% gelatin, pH 7.4.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)