Analysis Note

Positive ControlA549 cells or most tissues

Application

Immunoblotting (2 g/ml)Immunofluorescence (1 g/ml)Immunoprecipitation (1 g/reaction, see application references)Paraffin Sections (2.5 g/ml, heat pre-treatment required)

General description

This Anti-MDM2 (Ab-2) Mouse mAb (2A10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of MDM2 (Ab-2).

Recognizes the ~90 kDa (apparent MW) MDM2 protein in A549 cells.

Purified mouse monoclonal antibody (see application references). Recognizes the ~90 kDa (apparent MW) MDM2 protein.

Immunogen

human MDM2 protein

Epitope: within amino acids 294-339

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Marchetti, A., et al. 1995. J. Pathol.175, 31.Barak, Y., et al. 1993. EMBO. J.12, 461.Chen, J., et al. 1993. Mol. Cell Biol.13, 4107.Ladanyi, M., et al. 1993. Cancer Res.53, 16.Leach, F.S., et al. 1993. Cancer Res.53, 2231.Oliner, J.D., et al. 1993. Nature362, 857.Momand, J., et al. 1992. Cell69, 1237.Oliner, J.D., et al. 1992. Nature358, 80.Fakharzadeh, S.S., et al. 1991. EMBO. J.10, 1565.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)