Analysis Note
Positive ControlHBL-100 or SK-BR3 cells
Application
Frozen Sections (2 µg/ml)r>Immunoblotting (2 µg/ml, see application references)r>Immunofluorescence (2 µg/ml)r>Immunoprecipitation (2 µg, see application references)r>Paraffin Sections (not recommended)
General description
Anti-BRCA1 (Ab-4), mouse monoclonal, clone SD118, recognizes the ~220 kDa BRCA1 in HBL-100 and SK-BR3 cells. It is validated for WB, IF, IP, and for frozen sections.
Purified mouse monoclonal antibody generated by immunizing RBF/DnJ mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~220 kDa BRCA1 protein.
Recognizes the ~220 kDa BRCA1 protein in HBL-100 and SK-BR3 cells. Sold under license of U.S. Patent 5,753,441 and 6,162,897.
Immunogen
Human
a recombinant polypeptide corresponding to amino acids 1005-1313 in exon 11 of human BRCA1
Legal Information
Sold under license of U.S. Patents 5,753,441 and 6,162,897.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Maximal BRCA1 levels are observed in subconfluent cell populations after serum stimulation. Highly specific BRCA1 antibody useful for blotting and IP of full length BRCA1 protein. Antibody should be titrated for optimal results in individual systems.
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Packaging
100 µg in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Warning
Toxicity: Standard Handling (A)