Analysis Note

Positive ControlNIH/3T3 cells

Application

Immunoblotting (2.5 µg/ml, see application references)r>Immunoprecipitation (1 µg antibody or use Cat. No. OP07A; see application references)r>Frozen Sections (not recommended)r>Immunofluorescence (not recommended)r>Paraffin Sections (not recommended)

General description

Recognizes the ~60 kDa v-Src and c-Src proteins in NIH/3T3 and HT1080 cells. May appear as a doublet due to phosphorylation.

This Anti-v-Src (Ab-1) Mouse mAb (327) is validated for use in Immunoblotting, Immunoprecipitation, Frozen Sections, Immunofluorescence, Paraffin Sections for the detection of v-Src (Ab-1).

Purified mouse monoclonal generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X63 Ag8.653 myeloma cells (see application references). Recognizes the ~60 kDa Src protein.

Immunogen

purified v-Src

Epitope: within the SH3 domain of v-Src and c-Src

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

pp60^src may appear as a doublet due to phosphorylation. This antibody can be used to precipitate active src kinase (see application references). The agarose conjugate, Cat. No. OP07A, is available separately for immunoprecipitation. Antibody should be titrated for optimal results in individual systems.

Courtneidge, S.A. and Heber, A. 1987. Cell50, 1031.Cartwright, C.A., et al. 1985. Mol. Cell. Biol.5,, 2647.Courtneidge, S.A. 1985. EMBO J.4, 1471.Whitman, M., et al. 1985. Nature315, 239Bolen, J.B., et al. 1984. Cell38, 767.Hunter, T. and Sefton, B. 1980. Proc. Natl. Acad. Sci.77, 1311.Collett, M.S. et al. 1979. Proc. Natl. Acad. Sci.76, 3159.Collett, M.S. and Erikson, R.L. 1978. Proc. Natl. Acad. Sci.75, 2021.Collett, M.S., et al. 1978. Cell15, 1363.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.4.

Warning

Toxicity: Standard Handling (A)