Analysis Note

Positive ControlSW 480 or Y1 cells or normal skin tissue

Application

Immunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments)r>Other Applications (see comments)

General description

Rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Conjugated to agarose. Recognizes the ~21 kDa ras protein (see comments).

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate is validated for use in Immunoprecipitation, Other Applications for the detection of v-H-Ras (Ab-1).

Recognizes the ~21 kDa ras protein in SW480 and Y1 cells and normal skin tissue. For neutralization studies, Cat. No. OP01L is recommended. For immunoprecipitation, Cat. No. OP01A is recommended.

Immunogen

recombinant p21 Ras

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA80, 2112.Taparowsky, E., et al. 1983. Cell34, 581.Ellis, R.W., et al. 1981. Nature292, 506.Shih, T.Y., et al. 1980. Nature287, 686.

Agarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.

Packaging

.5 ml in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Warning

Toxicity: Standard Handling (A)