Analysis Note

Positive ControlProteins containing a His Tag™ sequence

Application

ELISA (0.1-0.4 g/ml)Immunoblotting (1-4 g/ml)Immunofluorescence (1-4 g/ml)Immunoprecipitation (4 g/ml)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes a His Tag™ sequence in native and recombinant proteins. Specifically recognizes an epitope of six consecutive His residues of both natural and recombinant sources.

Specifically recognizes an epitage of six consecutive His residues of both natural and recombinant sources.

Anti-Histidine-Tagged Protein, mouse monoclonal, clone 13/45/31/2, specifically recognizes an epitage of 6 consecutive His residues. It is validated for use in ELISA, WB, IF & IP.

Immunogen

a recombinant protein containing a His Tag™ sequence

Epitope: HHHHHH

Legal Information

Manufactured by Dianova, GmbH, Germany.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Zentgraf, H., et al. 1995. Nucl. Acids. Res.23, 3347.Gu, J., et al. 1994. BioTechniques17, 257.Sporeno, E., et al. 1994. J. Biol. Chem., 10991.Sisk, W.P., et al. 1994. J. Virol.68, 766.Lu, T., et al. 1993. Anal. Biochem.213, 318.Garner, J., et al. 1992. Cell69, 833.Hochuli, E., et al. 1987. J. Chromatogr.411, 177.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)