Analysis Note

Positive ControlRat brain hippocampus

Application

Frozen Sections (1 g/ml, see application references)Immunoblotting (1 g/ml)Immunoprecipitation (see comments)

General description

Recognizes the ~565 kDa ryanodine receptor in rat brain hippocampus. A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates.

This Anti-Ryanodine Receptor Mouse mAb (C3-33) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation for the detection of Ryanodine Receptor.

Mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3X 63 AG8.653 mouse myeloma cells (see application references). Supplied as protein G purified antibody. Recognizes the ~565 kDa ryanodine receptor.

Immunogen

Canine

canine cardiac ryanodine receptor (RYR)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Sitsapesan, R., et al. 1995. Trends Pharmacol. Sci.16, 386.McPherson, P.S. and Campbell, K.P. 1993. J. Biol. Chem.268, 13765.Meszaros, L.G., et al. 1993. Nature364, 76.Lai, F.A., et al. 1992. Biochem. J.288, 553.Lai, F.A., et al. 1989. Nature331, 315.

A ~565 kDa doublet representing the α and β isoforms is observed in non-mammalian vertebrates. In frozen sections, Clone C3-33 strongly stains the myofiber consistent with the sarcoplasmic reticulum of the RyR. This antibody has been reported to work for immunoprecipitation; the concentration is assay-dependent. Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)