Analysis Note
Positive ControlBrain tissue, serum
Application
ELISA (see comments)Immunoblotting (1:100-1:1000)Immunoprecipitation (see comments)Frozen Sections (1:100-1:10,000)Paraffin Sections (1:100-1:10,000, formic acid or heat pre-treatment required)
General description
Mouse monoclonal antibody supplied as diluted culture supernatant. Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E.
Anti-Apolipoprotein E, mouse monoclonal, clone E6D7, recognizes the ~35 kDa Apo E2, E3, and E4 in human serum and brain. It is validated for ELISA, WB, IP, and paraffin and frozen sections.
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum and brain.
Immunogen
a synthetic peptide corresponding to amino acids surrounding the polymorphic amino acid position 158 of ApoE
Human
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Recognizes the ~35 kDa E2, E3, and E4 isoforms of apolipoprotein E in human serum, but multiple bands are seen when used with brain tissue samples. Staining of formalin-fixed paraffin-embedded tissues may be enhanced by the pre-treatment of tissue with 70% formic acid for 10-30 min at room temperature or by heat pre-treatment using citrate buffer, pH 6.0. This antibody has also been reported to work for immunoprecipitation and ELISA. Antibody should be titrated for optimal results in individual systems.
Golabek, A.A., et al. 1996. J. Biol. Chem.271, 10602.
Packaging
100 µL in Plastic ampoule
Please refer to vial label for lot-specific concentration.
Physical form
Culture supernatant diluted in PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: