Analysis Note

Positive ControlA431, HeLa, or ML-1 cells

Application

Frozen Sections (see application references)r>Immunoblotting (1 µg/ml)r>Immunofluorescence (1-5 µg/ml)r>Immunoprecipitation (1 µg/sample)r>Paraffin Sections (1-5 µg/ml, heat pre-treatment required)

General description

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the p300 protein.

Recognizes the p300 protein in A431, HeLa, and ML-1 cells.

This Anti-p300 Mouse mAb (NM-11) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin Sections for the detection of p300.

Immunogen

Epitope: within amino acids 2071-2091 near the CBP C-terminus

Human

recombinant, human p300 protein

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Dallas, P.B., et al. 1997. Hybridoma16, 273. Shisler, J., et al. 1996. J. Virol.70, 68.Wang, H.G., et al. 1995. J. Virol.69, 7917. Banerjee, A.C.,et al. 1994. Oncogene9, 1733.Eckner, R., et al. 1994. Genes Dev.8, 869.Abraham, S.E., et al. 1993. Oncogene8, 1639.Wang, H.G., et al. 1993. J. Virol.67, 476.Howe, J.A., and Bayley, S.T., 1992. Virology186, 15.Rikitake, Y., and Moran, E., 1992. Mol. Cell. Biol.11, 2826.Yaciuk, P., and Moran, W., 1991. Mol. Cell. Biol.11, 5389.Egan, C., et al. 1988. Mol. Cell. Biol.8, 3955.Yee, S. and Branton, P., 1985. Virology147, 142.

Antibody should be titrated for optimal results in individual systems.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)