Anti-Inntag# 6(IT6),clR19/4-11/15

Application

Western Blotting Analysis: A representative lot detected Inntag 6 (IT6) in Western Blotting applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunoprecipitation Analysis: A representative lot detected Inntag 6 (IT6) in Immunoprecipitation applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Immunofluorescence Analysis: A representative lot detected Inntag 6 (IT6) in Immunofluorescence applications (Georgieva, M.V., et. al. (2015). Nat Methods. 12(10):955-8).

Anti-Inntag 6 (IT6), clone R19/4-11/15 is a mouse monoclonal antibody that detects the Innocuous Inntag IT6 wherein the tag protein is a fragment of Hev b 6.02 of Hevea brasiliensis. It has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Research CategorySecondary & Control Antibodies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Pro-hevein (UniProt: P02877; also known as Major hevein) is encoded by the HEV1 gene in Hevea brasiliensis (Para rubber tree). Pro-hevein is a N-acetyl-D-glucosamine / N-acetyl-D-neuraminic acid binding lectin that can inhibit fungal growth. It is known to cause allergic reaction in human. It contains one barwin domain and one chitin-binding type-1 domain. Inntag 6 IT6 is a small protein domain of Hevea brasiliensis that displays strong structural determinants and does not generate solubility issues in vivo and in vitro. Also, it is not shown to compromise cell fitness when overexpressed and has no detectable functional and localization effects when fused to a variety of target proteins. Unlike the monomeric HA, Flag and MYC tags, Inntag IT6 does not increase the intrinsic variability of critical size in budding yeast cells. Structure of the Inntag IT6 is based on a packed beta-sheet conformation.

Immunogen

His-tagged recombinant peptide fragment corresponding to 43 amino acids from Hevea brasiliensis.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG2b in PBS without azide.

Format: Purified

Quality

Evaluated by Western Blotting in transfected lysates from HEK293cells transfected with GFP-IT6.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Inntag 6 (IT6) in 10 µg lysate from HEK293 cells transfected with GFP-IT6.

Specificity

Clone R19/4-11/15 detecs Pro-hevein in HEK293 cells tranfected with GFP-IT6.

Storage and Stability

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~34.1 kDa observed; 21.86 kDa calculated. Uncharacterized bands may be observed in some lysate(s).