Analysis Note

ControlMouse bone marrow cells

Application

Detect mouse CD44 (H-CAM) using this Anti-mouse CD44 (H-CAM) Antibody, clone KM201 validated for use in FC.

Research CategoryCell StructureStem Cell Research

Research Sub CategoryAdhesion (CAMs)Hematopoietic Stem Cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

CD44, also known as H-CAM is a distinct adhesion molecule that, with its 13 known isoforms, comprises a family of transmembrane glycoproteins involved in a wide variety of significant biological processes. Members belonging to this family are involved in apoptosis, migration, metastasis, tumor progression, lymphocyte homing, and the attachment of hematopoietic cells to stromal cells. CD44 is the primary cell surface receptor for hyaluronic acid (HA), and is also a notable target for WNT signaling within the intestinal mucosa. Recent studies have used CD44 marker as a means to isolate different cancer stem cells (CSC) such as; colorectal CSC, pancreatic CSC, and prostate CSC.

Immunogen

Mouse bone marrow-derived stromal cells

Epitope: Near the hyaluronate binding domain

Linkage

Replaces: CBL1308

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G

Format: Purified

Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

Quality

Evaluated by Flow Cytometry in mouse bone marrow cells.

Flow Cytometry Analysis: 0.1 µg of this antibody detected CD44 in mouse bone marrow cells.

Specificity

The antibody recognizes CD44 near the hyaluronate binding domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

86 kDa calculated