Anti-MLC-2; clone 19D3.1

Analysis Note

ControlHuman skeletal muscle tissue lysate.

Application

Research CategoryCell Structure

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MLC-2 in human cardiac myocyte and in rat skeletal muscle tissues.

Detect Myosin using this mouse monoclonal antibody, Anti-MLC-2 Antibody, clone 19D3.1 validated for use in western blotting & IHC.

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Myosin light chain 2 (MLC2), also known as myosin regulatory light chain (MRLC), RLC, or LC20, has several isoforms and is a component of myosin, which is made up of two heavy chains and four light chains. MLC2 is involved in smooth muscle contraction, stress fiber assembly, and cytokinesis. MLC2 defects can cause of familial hypertrophic cardiomyopathy type 10 (CMH10).

Immunogen

GST-tagged recombinant protein corresponding to human MLC-2.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:2,000 dilution from a representative lot detected MLC-2 in 10 µg of human skeletal muscle tissue lysate.

Specificity

Other homologies: Mouse 92% sequence homologies.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~19 kDa observed