Analysis Note

ControlSW1353 cell lysate

Application

Detect the Osterix (SP7) protein using this Anti-Osterix (SP7), clone 6B12.1 validated for use in WB & IHC.

Research CategoryCell Structure

Immunohistochemistry Analysis: A 1:200 dilution from a representative lot detected Isocitrate dehydrogenase in rat tooth tissue. Nuclear staining may be observed in epithelial cells in some tissues.

Research Sub CategoryCytoskeletal Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

OSTERIX, or SP7, is a zinc finger-containing, osteoblast-specific transcription factor that is distinctly expressed in all developing bones, and is a key requirement for the differentiation of pre-osteoblasts into fully functional osteoblasts. While research on the actual functional mechanisms of OSTERIX is still on-going, current findings indicate that OSTERIX expression is induced by BMP-2 and Dlx5, and operates downstream of RUNX2, supporting its role in mesenchymal stem cell differentiation. Further studies also indicate that the OSTERIX transcription factor may also play a role in negatively regulating the Wnt/β-catenin signaling pathways and the proliferation of osteroblast cell types during embryonic osteogenesis.

Immunogen

GST-tagged recombinant protein corresponding to human Osterix.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in SW1353 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Osterix in 10 µg of SW1353 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed. An uncharacterized band may appear at ~160 kDa in some lysates.