ANTI-ZNF175 (OTK18); CLONE 211-3.1-1

Application

ELISA Analysis: A representative lot detected OTK18(ZNF175) in ELISA applications (Buescher, J.L., et. al. (2008). J Neuroimmune Pharmacol. 3(4):230-5).

Anti-OTK18(ZNF175) clone 211 3.1 1, Cat. No. MABT1491, is a mouse monoclonal antibody that detects Zinc finger protein 175 (OTK18) and has been tested for use in ELISA and Immunocytochemistry.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Zinc finger protein 175 (UniProt: Q9Y473; also known as Zinc finger protein OTK18) is encoded by the ZNF175 gene (Gene ID: 7728) in human. ZNF175 is zinc finger protein that contains 13 C2H2 type zinc fingers and is shown to be ubiquitous in its expression. ZNF175 displays antiviral activity and it is produced during HIV-1 infection of mononuclear phagocytes in human and interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity. It down-regulates the expression of several chemokine receptors and plays a role in the interaction of viral and host cell factors leading to HIV-1 associated dementia and HIV-1 encephalitis. ZNF175 contains a Krüppel associated box (KBAB; aa 26-89) domain that is responsible for its repressive activity. Plasma levels of ZNF175 are reported to correlate with CD8 count and HIV status. (Ref.: Buescher, J., et al. (2008). J Neuroimmune Pharmacol. 3(4); 230-235).

Immunogen

Epitope: N-terminus

His-tagged recombinant fragment corresponding to the first 178 amino acids from the N-terminal region of human Zinc finger protein 175.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected OTK18(ZNF175) in HeLa cells.

Specificity

Clone 211 3.1 1 specifically detects human Zinc finger protein 175. It targets an epitope with in the first 178 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

81.61 kDa calculated.