Anti-Protein Spindly; clone 9D10.2

Analysis Note

ControlHEK293 cell lysate

Application

Research Sub CategoryCytoskeleton

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Protein Spindly in HeLa and NIH/3T3.

Research CategoryCell Structure

Detect Protein Spindly using this Anti-Protein Spindly Antibody, clone 9D10.2 validated for use in ICC, Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein spindly, otherwise known as the coiled-coil domain-containing protein 99 (CCDC99), is a phosphoprotein that regulates mitosis. It interacts with the Rod/ZW10/Zwilch complex, and is involved in recruiting, docking, and alignment of motor protein dynein at mitotic kinetochores. Spindly may also be involved in checkpoint signaling and microtubule attachment.

Immunogen

GST-tagged recombinant protein corresponding to human Protein Spindly.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified by Ion-Exchange Chromatography

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Protein Spindly in 10 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~70 kDa observed. This protein is known to exist in two isoforms at 70 kDa and 56 kDa An uncharacterized band may be observed at ~38 kDa in some cell lysates.