Application

Research CategorySignaling

This Anti-Anoctamin-5 Antibody, clone 5F7 is validated for use in Western Blotting for the detection of Anoctamin-5.

Research Sub CategorySignaling Neuroscience

Western Blotting Analysis: A 1:100 dilution from a representative lot detected Anoctamin-5 in 10 µg of mouse leg muscle tissue lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Anoctamin-5 (UniProt Q75V66; also known as Gnathodiaphyseal dysplasia 1 protein, Integral membrane protein GDD1, Transmembrane protein 16E) is encoded by the ANO5 (also known as GDD1, LGMD2L, TMEM16E) gene (Gene ID 203859) in human. The human Anoctamins (ANO) compose a family of at least ten proteins, all exhibiting eight transmembrane domains and a DUF590 domain of unknown function. The ANO1, ANO2, ANO6, ANO8, and ANO9 are characterized to code for the elusive calcium-activated chloride channels (CaCCs). While the physiological function of anoctamin-5 is not yet characterized, dominant mutations in ANO5 have been found in the rare skeletal disorder gnathodiaphyseal dysplasia. In addition, recessive mutations in ANO5 are found to cause proximal muscular dystrophy (LGMD2L) and distal Miyoshi myopathy (MMD3).

Immunogen

Linear peptide corresponding to mouse Anoctamin-5 near the C-terminus.

Epitope: Near C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Format: Unpurified

Mouse monoclonal IgMκ in buffer containing supernatant without preservatives.

Unpurified

Quality

Evaluated by Western Blotting in mouse skeletal muscle tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Anoctamin-5 in 10 µg of mouse skeletal muscle tissue lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~37 kDa observed. ~37 kDa observed. Although the full-length protein has a calculated molecular weight of 107.2 kDa (Uniprot Q75V66), only a ~37 kDa band corresponding to a short spliced variant can be detected by Western blotting when using muscle tissue lysates. A band corresponding to the full-size protein and a 55 kDa band can be detected in addition to the 37 kDa band when using lysates prepared from human embryonal rhabdomyosarcoma (RMS) RD cells, C2C12 murine myoblasts, and fibroblasts.