Anti-Sclerostin; clone 7B6.1

Application

Research CategorySignaling

Research Sub CategoryDevelopmental Signaling

Anti-Sclerostin Antibody, clone 7B6.1 | MABS445 is an antibody against Sclerostin Antibody for use in Western Blotting and Immunohistochemistry.

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Sclerostin in human kidney, rat liver and human liver tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sclerostin (UniProt Q9BQB4) is encoded by the SOST (also known as CDD, SOST1, VBCH) gene (ORP UNQ2976/PRO7455/PRO7476; Gene ID 50964) in human. Sclerostin is a glycoprotein expressed in osteocytes and functions as a negatively regulator against bone mass by antagonizing low density lipoprotein receptor-related proteins- (LRP5-/LRP6-) mediated WNT/beta-catenin pathway signaling. Sclerostin levels are increased in the post-menopausal women and elderly and in long term immobilized patients. Sclerostin neutralizing antibody is reported to enhance bone formation, mass, and strength in aged male rats. Sclerostin and its inhibitors have potential in prosthetic, regenerative and preventive therapy in dentistry.

Immunogen

GST-tagged recombinant protein corresponding to the internal fragment of human Sclerostin.

Epitope: Internal

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Mouse monoclonal IgG2bκ antibody tissue culture supernatant without preservatives.

Unpurified

Quality

Evaluated by Western Blotting in GST-tagged Sclerostin recombinant protein.

Western Blotting Analysis: A 1:500 dilution of this antibody detected 0.5 µg of GST-tagged Sclerostin recombinant protein.

Specificity

Detects Sclerostin isoform 1 (UniProt Q9BQB4-1). Reactivity toward the spliced isoform 2 (UniProt Q9BQB4-2) is likely, but has not been determined.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

24 kDa calculated