Anti-MEK1; clone 6B7.1

Application

Research CategorySignaling

Anti-MEK1 Antibody, clone 6B7.1 is an antibody against MEK1 for use in Western Blotting.

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dual specificity mitogen-activated protein kinase kinase 1 (EC 2.7.12.2; UniProt Q02750; also known as ERK activator kinase 1, MAP kinase kinase 1, MAPK/ERK kinase 1, MAPKK 1, MEK 1, MKK1) is encoded by the MAP2K1 (also known as CFC3, MAPKK1, MEK1, MKK1, PRKMK1) gene (Gene ID 5604) in human. MEK1 and the related MEK2 are dual specificity kinases of the MAP2K/STE7 kinase family. MEK1 and MEK2 share a high degree of sequence homology and perform similar functions. Upon activation by RAF, MEK1/2 catalyze the dual phosphorylation of threonine and tyrosine residues on ERK (MAPK) within a specific Thr–Glu–Tyr sequence at codons 202 to 204. This locks the kinase domain of MAPK into a catalytically competent conformation, ultimately leading to nuclear signals that regulate cellular growth and proliferation. MEK1/2 mutations have been reported to be associated with cardiofaciocutaneous syndrome 3 (CFC3) and cancers, including lung cancer, colorectal cancer, and melanomas.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminal region of human MEK1.

Epitope: C-terminal region

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MEK1 in 10 µg of HeLa cell lysate.

Specificity

Expected to react with both the MKK1a and MKK1b spliced isoforms.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed