Application

Research CategorySignaling

Immunohistochemistry (Paraffin) Analysis: A representative lot detected HSD3B2 in Immunohistochemistry appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Western Blotting Analysis: Analysis: A representative lot detected HSD3B2 in Western Blotting appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Immunohistochemistry (Paraffin) Analysis: A 1:50-250 dilution from a representative lot detected HSD3B2 in human adrenal gland and human placenta tissue sections.

ELISA Analysis: A representative lot detected HSD3B2 in ELISA appliations (Gomez-Sanchez, C.E., et al. (2017). Steroids. 127:56-61).

Anti-HSD3B2, clone 6, Cat. No. MABS2067, is a mouse monoclonal antibody that detects 3-beta-HSD II (HSD3B2) and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. (3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (UniProt: P26439; also known as 3 beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type II, 3-beta-HSD II, 3-beta-HSD adrenal and gonadal type) is encoded by the HSD3B2 (also known as HSDB3B) gene (Gene ID: 3284) in human. Humans have two HSD3B isozymes. HSD3B1 is mainly expressed in the placenta, mammary and, prostate glands, and other peripheral tissues including the skin and adipose tissue and HSD3B2 is expressed primarily in the adrenal and gonads. They exhibit about 93% sequence homology. 3-beta-HSD II is a single-pass membrane protein found on mitochondrial and endoplasmic reticulum membranes. It is expressed in adrenal gland, testis and ovary. It is a bifunctional enzyme that catalyzes the oxidative conversion of Delta 5-ene-3-beta-hydroxy steroid and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. Mutations in HSD3B2 gene are reported to cause adrenal hyperplasia that is characterized by androgen excess, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormone hypersecretion (Ref.: Gomez-Sanchez, CE et al. (2017). Steroids 127; 56-61).

Immunogen

KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal region of human 3-beta-hydroxysteroid dehydrogenase/Delta 5→4-isomerase type 2 (3-beta-HSD).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human adrenal gland tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected HSD3B2 in human adrenal gland tissue lysate.

Specificity

Clone 6 specifically detects HSDB32 in human tissue. it targets an epitope with in 14 amino acids from the N-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed; 42.05 kDa calculated. Uncharacterized bands may be observed in some lysate(s).