Anti-Dach1; clone 1F6.1

Application

Research CategorySignaling

Anti-Dach1, clone 1F6.1, Cat. No. MABS1805, is a mouse monoclonal antibody that detects Dach1 in human and murine cells and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dach1 in human bone marrow and human colon cancer tissues.

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Dach1 in Neuro-2a cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dachshund homolog 1 (UniProt: Q9UI36; also known as Dach1) is encoded by the DACH1 (also known as DACH) gene (Gene ID: 1602) in human. Dach1 is a transcription factor that is involved in regulation of organogenesis. Four isoforms of Dach1 have been reported that are produced by alternative splicing. It is shown to be widely expressed. Isoform 2 is found in brain, heart, kidney, liver, leukocytes and spleen. Isoform 3 is found in liver and heart and isoform 4 is found in spleen. Clone 1F6.1 exhibits reactivity with all four isoforms. Dach1 is reported to act as regulator of SIX1, SIX6, and probably SIX5. It also acts as a a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Dach1 is shown to Inhibit TGF-beta signaling through interaction with SMAD4 and NCOR1. It binds to chromatin DNA via its DACHbox-N domain, which forms a structure containing a DNA binding motif similar to that of the forkhead/winged helix domain. PELP1 (proline, glutamic acid, and leucine rich protein 1) serves as a Dach1-binding protein and the carboxyl terminus of Dach1 is required for binding to PELP1. Dach1 is reported to prevent the proliferation of cancerous cells due to being a natural repressor of estrogen receptors (ER) in the normal function of mammary tissue. It inhibits ER alpha transcriptional activity. (Ref.: Popov, VM et al. (2009). Cancer Res. 69(14); 5752-60). +D1

Immunogen

GST/His-tagged recombinant fragment coreesponding to 60 amino acids from the C-termnal region of human Dach1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Format: Purified

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in SHSY-5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Dach1 in 10 µg of SHSY-5Y cell lysate.

Specificity

Clone 1F6.1 detects all isoforms of Dach1 in human and murine cells. It targets an epitope within 60 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~78 kDa observed; 78.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).