ANTI-HSD17B3, CLONE 3E11.2

Application

Research CategorySignaling

Detect HSD17B3 using this mouse monoclonal Anti-HSD17B3 Antibody, clone 3E11.2, Cat. No. MABS1165, validated for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Testosterone 17-beta-dehydrogenase 3 (EC 1.1.1.64; UniProt P37058; also known as 17-beta-HSD 3, 17-beta-hydroxysteroid dehydrogenase type 3, Short chain dehydrogenase/reductase family 12C member 2, Testicular 17-beta-hydroxysteroid dehydrogenase) is encoded by the HSD17B3 (also known as EDH17B3, SDR12C2) gene (Gene ID 3293) in human. 17 -hydroxysteroid dehydrogenases (17 -HSDs) mediate the activation and deactivation of sex steroids involving redox reactions centered about the C17 area of the steroid backbone. 17 -HSD3 catalyzes the final step in the steroidogenesis of the potent androgen testosterone (T) by selectively reducing the C17 ketone of 4-dione, utilizing NADPH as a cofactor, in testis and prostate. Upregulated 17 -HSD3 mRNA level is found in cancerous prostate biopsies when compared to normal tissue, while 17 -HSD3 deficiency caused by HSD17B3 gene mutations results in 46,XY Disorders of Sex Development (46,XY DSD).

Immunogen

KLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human HSD17B3.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified.

Format: Purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in mouse testis tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HSD17B3 in 10 µg of mouse testis tissue lysate.

Specificity

Clone 3E11.2 targets an epitope within the N-terminal half of mouse HSD17B3 and the two human spliced isoforms reported by UniProt (P70385 and P37058).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed. 34.52/29.11 kDa (human isoform 1/2) and 34.33 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).