Application

This Anti-DARPP-32 Antibody, clone 9B6.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of DARPP-32.

Research Sub CategorySignaling Neuroscience

Research CategoryNeuroscience

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected DARPP-32 in 10 µg of rat brain tissue lysate.
Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected DARPP-32 in human cerebral cortex and human testis tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein phosphatase 1 regulatory subunit 1B (EC 3.1.3.16; UniProt Q9UD71; also known as DARPP-32, Dopamine- and cAMP-regulated neuronal phosphoprotein 32) is encoded by the PPP1R1B (also known as DARPP32) gene (Gene ID 84152) in human. DARPP-32 is a PKA substrate that, upon phosphorylation on Thr34 by PKA, functions as a protein-phosphatase 1 (PP1) inhibitor. DARPP-32 N-terminal a.a. 8-11 (KIQF) is essential for PPI binding. DARPP-32 is an important mediator of dopamine receptor-dependent NMDA receptor activation. Dopamine receptor activation leads to cAMP induction, which activates cellular PKA activity and subsequent DARPP-32 Thr34 phosphorylation. PP1 inhibition by the Thr34 phosphorylated DARPP-32 in turn results in an enhnaced NMDA receptor phosphorylation. Calcium/calmodulin-dependent protein phosphatase 2B (PP2B or calcineurin) can reverse DARPP-32 Thr34 phosphorylation and negatively regulate its PP1 inhibitory activity. DARPP-32 is also known to be phosphorylated on Ser137 by casein kinase I (CKI), on Ser102 by CKII, and on Thr75 by Cdk5. Ser137 phosphorylation prevents PP2B from dephosphorylating pSer34, and Ser102 phosphorylation promotes Thr34 phosphorylation by PKA. Thr75 phosphorylated DARPP-32 is reported to exhibit PKA inhibitory activity in vitro.

Immunogen

GST-tagged recombinant protein corresponding to the C-terminal half of human DARPP-32.

Epitope: C-terminal half.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected DARPP-32 in 10 µg of human brain tissue lysate.

Specificity

Expected to react with both spliced isoforms of human DARPP-32 (UniProt Q9UD71-1 & Q9UD71-2).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~32/30 kDa observed. Target band(s) appears larger than the caluclated molecular weights of 22.96 kDa (isoform 1) and 18.74 kDa (Isoform 2).