Anti-CRALBP; clone 3H9.1

Application

Research CategoryNeuroscience

Research Sub CategorySensory & PNS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Retinaldehyde-binding protein 1 (UniProt P12271; also known as Cellular retinaldehyde-binding protein) is encoded by the RLBP1 (also known as CRALBP) gene (Gene ID 6017) in human. CRALBP is a retinoid-binding protein expressed in retinal pigment epithelial (RPE) cells and Müller glia involved in the retinal visual cycle. Photoactivation of a visual pigment molecule in vertebrate rod and cone photoreceptors renders the pigment molecule into a bleached all-trans retinal form, which must be exported out of the photoreceptors to be converted back to the light-sensitive 11-cis retinal form in RPE cells (for both rods and cones) or in retinal Müller glia (for cones only). The 11-cis chromophore is then imported back into photoreceptors, where it combines with a free opsin to regenerate the visual pigment. CRALBP is a 36 kDa water-soluble protein that facilitates the intracellular transport of hydrophobic 11-cis retinoids. Mutations in human RLBP1 cause several autosomal recessive retinal diseases, such as retinitis pigmentosa, Bothnia dystrophy, retinitis punctata albescens, fundus albipunctatus, and Newfoundland rod-cone dystrophy. Likewise, Rlbp1-knockout mice exhibit M-opsin mislocalization, M-cone loss, and impaired cone-driven visual behavior and light responses. Viral expression of CRALBP in Müller cells, but not RPE cells, is reported to rescue the retinal visual cycle and M-cone sensitivity in Rlbp1-knockout mice.

Immunogen

Epitope: N-terminal half.

GST-tagged recombinant protein corresponding to the N-terminal half of human CRALBP.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in mouse retina tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected CRALBP in 10 µg of mouse retina tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~36 kDa observed.