Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.
Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected phospho Drebrin (Ser142) in HeLa and NIH/3T3 cells.
Peptide Inhibition Analysis: 0.5 µg/mL from a representative lot detected phospho Drebrin (Ser142) in 10 µg of rat neonatal brain tissue lysate.
Immunocytochemistry Analysis: A representative lot detected phospho Drebrin (Ser142) in rat embryonic cortical culture (Prof. Phillip R. Gordon-Weeks, King′s College London).

Anti-phospho Drebrin (Ser142), clone 3C14 | MABN833 is an antibody against phospho Drebrin for use in Western Blotting, Immunocytochemistry, Peptide Inhibition Assay.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Developmentally-regulated brain protein, also known as Drebrin, and encoded by the gene name DBN1, is a developmentally-regulated neuron-specific protein, expressed during outgrowth and maturation of dendrites, and localized within dendrites of the adult brain. Drebrin is required for actin polymerization at immunological synapses (IS) and for CXCR4 recruitment to IS. Recent studies have shown that Drebrins may play a role in cell migration, extension of neuronal processes and plasticity of dendrites. Drebrins are classified into two forms of the embryonic type (E1 and E2) and one form of the adult type (A). The time course of their appearance are different from each other. Their structures are closely related. Adult rat brain expresses only drebrin A while drebrin E1 or E2 is observed in immature animals. Drebrin E+A- signals have been observed in the cell bodies of migrating neuroblasts in the core region; however, only fibrous and punctate drebrin E+A- signals have been observed in postmigratory neuroblasts at the outer layers. Studies have demonstrated that the disappearance of Drebrin E+A- signals from the cell body coincides with the cessation of neuronal migration. The disappearance of Drebrin E from the cell body may be a molecular switch for the cessation of migration in newly generated neuroblast. Drebrin is expressed mainly in brain neurons.

Immunogen

KLH-conjugated linear peptide corresponding to rat phospho Drebrin (Ser142).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in SH-SY5Y cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected phospho Drebrin (Ser142) in 10 µg of SH-SY5Y cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~100 kDa observed. The calculated molecular weight is 71 kDa, however phospho Drebrin (Ser142) has been shown as a ~100 kDa band in western blots (Worth, D.C., et al. (2013). JCB. 202(5):793-806).