Anti-EYS; clone 3G10.1

Application

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected EYS in human and rat retina tissue sections.

Research CategoryNeuroscience

Anti-EYS Antibody, clone 3G10.1 is an antibody against EYS for use in Western Blotting, Immunohistochemistry (Paraffin).

Research Sub CategorySensory & PNS

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protein eyes shut homolog (UniProt Q5T1H1; also known as EGF-like protein 10, EGF-like protein 11, Epidermal growth factor-like protein 10, Epidermal growth factor-like protein 11, Protein spacemaker homolog) is encoded by the EYS (also known as C6orf178, C6orf179, C6orf180, EGFL10, EGFL11, SPAM) gene (ORF UNQ9424/PRO34591, Gene ID 346007) in human. Drosophila eyes shut protein is expressed in photoreceptor cells, the central nervous system, and peripheral sensory ganglia, and is essential for the formation of a matrix-filled intra-rhabdomeral space within the fly′s retina ommatidia, the loss of spacemaker (spam) converts an open rhabdom system to a closed one. Human EYS/SPAM gene likewise is found to be expressed in the eye and the spinal cord, and mutations in human EYS result in retinitis pigmentosa. Human EYS protein contains 27 EGF-like repeats and five laminin G-like (LG1-5) domains (between EGF repeats 21 & 21, 21 & 22, 23 & 24, 25 & 26, and after repeat EGF repeat 27). The presence of the EGF-like repeats is indicative of a role in the Notch signaling pathway, while EYS LG1-5 bear resemblance to the three agrin laminin G domains that are known to interact with several proteins, including α-dystroglycan at the muscle surface.

Immunogen

GST-tagged recombinant human EYS fragment corresponding to the EGF-like domains 9-11.

Epitope: EGF-like domains 9-11.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting of EYS recombinant protein.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected EYS recombinant protein.

Specificity

Expected to react with spliced isoforms 1 and 3, but not isoforms 2 and 4, reported by UniProt (Q5T1H1).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

348.4 kDa (isoform 1) and 350.8 kDa (isoform 3) kDa calculated