Application

Research CategoryNeuroscience

This Anti-Dual oxidase 2, clone Duox S-12 Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunocytochemistry for the detection of Dual oxidase 2.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Dual oxidase 2 in human prostate cancer and human breast cancer tissue.
Western Blotting Analysis: A representative lot detected Dual oxidase 2 in BxPC-3 cell lysate (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238).
Immunocytochemistry Analysis: A representative lot detected Dual oxidase 2 in MIA PACA-2 cell (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238).
Immunohistochemistry Analysis: A representative lot detected Dual oxidase 2 MIA PACA-2 tissue (Wu, Y., et al. (2013). International Journal of Oncology. 42:1229-1238).

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Dual oxidase 2 is an important enzyme in thyroid hormone biosynthesis and peroxide metabolism. Dual oxidase 2 generates the hydrogen peroxide needed for thyroid peroxidase and lactoperoxidase enzymes as well as processes H2O2 made during metabolic processes. Dual oxidase also plays a role in anti-microbial defense in our mucous membranes by providing the peroxidase for lactoperoxidase's antimicrobial activities. Dual oxidase 2 is also needed for various extracellular matrix stabilizations and protein cross-linking needs such as during cuticle formation. Dual oxidase 2 is widely expressed in the developing fetus, and in the adult, it is largely expressed in the mucosal and epithelial cells and linings of the gut, lung, salivary glands and testis. Dual oxidase also plays a role in many hypersensitivity and inflammatory diseases and is upregulated in diseases such as colitis and certain cancers.

Immunogen

Recombinant protein corresponding to the N-terminus of human Dual oxidase 2.

Epitope: N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in BxPC3 treated with IL-4 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dual oxidase 2 in 10 µg of BxPC3 treated with IL-4 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~175 kDa observed