Anti-Dopamine Transporter; clone mAb16

Analysis Note

ControlMouse brain tissue lysate

Application

Anti-Dopamine Transporter Antibody, clone mAb16 is a highly specific mouse monoclonal antibody & that targets Dopamine Transporter & has been tested in western blotting.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Western Blotting Analysis: A representative lot from an independent laboratory detected Dopamine Transporter in rat striatal membrane tissue lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The transmembrane dopamine transporter (DAT) is located on the presynaptic nerve terminal and is responsible for terminating dopaminergic transmission by transporting dopamine from the synaptic cleft into the dopaminergic neuron (reuptake). Dopaminergic pathways have been strongly implicated in reward and addiction, motivation, alcoholism, ADHD and degenerative motor diseases such as Parkinson′s, Huntington′s and Chorea. EMD Millipore's Anti-Dopamine Transporter antibody has been validated in western blotting for mouse and rat specimens.

Immunogen

Epitope: Cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of rat Dopamine Transporter.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in mouse brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Dopamine Transporter in 10 µg of mouse brain tissue lysate.

Specificity

This antibody recognizes the cytoplasmic domain of Dopamine Transporter.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 68 kDa observed