Analysis Note

ControlRat brain membrane tissue lysate

Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Signaling

Anti-Gs protein Antibody, alpha subunit, clone N192/12 is a highly specific mouse monoclonal antibody & that targets Gsα & has been tested in western blotting.

Western Blotting Analysis: 2.0 µg/mL from a representative lot detected GS protein, alpha subunit in 10 µg of SH-SY5Y cell lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The heterotrimeric guanine nucleotide-binding regulatory proteins (G proteins) mediate signaling from a large number of G-protein coupled receptors (GPCR) to a variety of intracellular effectors. G-proteins are composed of a 36-52 kDa α-subunit, a 35-36 kDa β-subunit, and an 8-10 kDa γ-subunit. They are classified based on the nature of their α-subunit. Gs is ubiquitously expressed and couples receptors to adenylyl cyclase and promotes intracellular cAMP generation. Mutations in Gs-α can lead to constitutive activation of receptors leading to development of endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. In brain tissue Gs-α activates adenyl cyclase in direct response to stimulation of several neurotransmitter receptors and facilitates depolarization-induced neurotransmitter release for synaptic transmission. Also, it is shown that Gs-stimulated adenylyl cyclase activity is significantly reduced in the Alzheimer′s disease brain tissue.

Immunogen

Recombinant protein corresponding to bovine GS protein, alpha subunit.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in rat brain membrane tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GS protein, alpha subunit in 10 µg of rat brain membrane tissue lysate.

Specificity

Other homologies: Mouse (99% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~45 kDa observed